Abstract
To enable photometrical pH measurements to be carried out by means of optical sensors using membrane-protected pH fluorescence indicators (optodes), a correction method for ionic strength-independent pH measurements has been developed. The correction principle is based on a double pH fluorescence indicator system which, simultaneously, allows a continuous determination of ionic strength, since ionic strength more or less influence the p K values of the pH indicators depending on their valence. Measurements were performed with the indicators hydroxypyrenetrisulfonic acid (valence, −4) and β-methylumbelliferone (valence, −1).
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