New flavone and eudesmane derivatives from Lawsonia inermis and their inhibitory activity against NO production

Abstract

A new flavone derivative, 7-hydroxy-3,5-dimethoxy-6,8-dimethylflavone (1) and a new eudesmane derivative, eudesmane-4β,7α-diol (2), have been isolated from the aerial part of Lawsonia inermis, together with ten known compounds (3–12). The structures of two new compounds were determined through spectroscopic and MS analyses. All compounds were evaluated for their inhibitory effects on Nitric Oxide production in LPS-stimulated RAW264.7 cells and compounds 3, 4, 6, 7, 9, and 10 showed inhibition with IC50 values of 8.11, 2.32, 1.87, 7.72, 2.18, and 6.34μg/mL, respectively.