New Fixation Technique for Preimplantation Genetics Diagnosis

Abstract

Objectives: Current fixation techniques for preimplantation genetic diagnosis present an embryologist with several problems. These include premature drying, resulting in excess of cytoplasm; scattering which may lead to specimen loss; and significant variation in the required duration of treatment with pepsin. As a result, blastomeres or polar body fixation is a cumbersome and stressful process that requires extensive training. The development of an easier and more reproducible fixation technique is an important goal that was addressed in the present study.