Abstract
New recombinant strains of Penicillium verruculosum have been created with a high level of expression of homologous endo-β-1,4-glucanase II and heterologous phytase A A. niger, as well as heterologous endo-1,4-β-xylanase E P. canescens and phytase A A. niger, allowing to obtain highly active feed enzyme preparations (AF) capable of simultaneously signi cantly reducing viscosity non-starch polysaccharides, as well as increase the bioavailability of phosphorus and minerals of grain-based feed and, thus, increase the digestibility of nutrients of agricultural animal and poultry feed. Speci c AF activities for speci c substrates (xylanau, β-glucan, phytate) were studied, the qualitative and quantitative component composition of new AF was determined. On the basis of a new enzyme preparation containing homologous endo-β-1,4-glucanase II and heterologous phytase A A. niger, a recipient strain ∆nia D - was obtained, into which heterologous endo-1,4-β-xylanase E P. canescens was successfully cloned and, thus, a promising producer of three target enzymes was obtained simultaneously.
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