Abstract
A new procedure for thorough extraction of lipids (designated AIB procedure) was developed during an investigation of lipid metabolism in Stephanurus dentatus, a nematode parasite of swine. Worm homogenates in 0.9% sodium chloride were shaken with 1 2 homogenate volume of acetone (A), and then, in addition, with 1 homogenate volume of the organic phase (IB) of a mixture of isopropanol, benzene, and water (2:2:1, v/v). After centrifugation, the organic (upper) layer was removed and the aqueous phase and interface extracted three additional times. Extracts were reduced in vacuo and maintained under nitrogen. Higher lipid yields were obtained by the AIB method as compared to the well-known Folch procedure. Recoveries for lipid phosphorus, lipid nitrogen, and free sterols are given. This method achieves a very thorough extraction of polar and nonpolar lipids and is especially useful for the extraction of tissue homogenates. It is suggested that this method may be applied to lipid extraction of a variety of biological preparations.
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