Abstract
A cDNA for a prostaglandin E2 (PGE2) receptor subtype 4, EP4b (Ptger4b), was cloned from the medaka ovary. The effect of PGE2 was examined using COS-7 cells expressing the recombinant Ptger4b protein. An increase in intracellular cAMP levels was observed when the cells were incubated with PGE2, but the increase in cAMP levels was nullified by the addition of the EP4 antagonist GW627368X. The expression of ptger4b mRNA was drastically induced by the addition of pregnant mare serum gonadotropin to the in vitro culture of large preovulatory follicles. In in vitro ovulation studies of the effect of GW627368X addition on follicle ovulation, the critical timing of the PGE2/Ptger4b interaction was suggested to be between −1 and 0h of ovulation. These results further substantiate that PGE2/Ptger4b signaling is involved in follicle rupture during ovulation in the medaka ovary.
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