Abstract
There have been several recent methods for determining serum oxalate in man (1-8), but reported normal values have ranged from 0.3 to 14 mg/liter. Recently Knowles and Hodgkinson (6) reported an enzymic method for oxalate determination in human serum by use of oxalate decarboxylase (EC 4.1.1.2) with subsequent colorimetric determination of the evolved CO2. The method is specific, but the technique is rather too elaborate for routine use. Our method also involves use of oxalate decarboxylase in a linked reaction with NAD+ requiring formate dehydrogenase (EC 1.2.1.2). Determinations are done spectrophotometrically, with reproducible results. We have recently applied the principle successfully for oxalate determinations in urine (9). In serum the mean value for data from 40 healthy volunteers was 1.82 mg/liter, the values for women being significantly higher than for men.
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