Abstract
The demand for enzymes in different industries is continuously increasing, and the use of a pure form of carbon and nitrogen source for enzyme production is expensive. Thus, organic waste as a low-cost substrate is used to produce enzymes, mitigate the market demand, and reduce the production cost. Besides, this action helps in decreasing organic waste and finally pollution. This study aims to utilize agro-waste for maximum multi-enzyme production by Streptomyces sp. and to formulate the three agro-waste mixtures using a statistical approach; extreme vertices mixture design (EVMD). The study illustrated the bacterium produced a maximum of 4.24 ± 0.05 U/mL pectinase, 2.88 ± 0.05 U/mL polygalacturonase (PGase), 9.43 ± 0.16 U/mL xylanase, and 1.62 ± 0.09 U/mL cellulase by formulating the agro-waste mixture of orange peel, pomegranate peel, and pumpkin pulp+seeds. Also, the mixture of agro-waste enhanced enzyme production rather than the single feedstock, and the enzyme production depends on the agro-waste mixture.
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