Abstract
BackgroundAcinetobacter baumannii is an important nosocomial pathogen that can develop multidrug resistance. In this study, we characterized the genome of the A. baumannii strain DMS06669 (isolated from the sputum of a male patient with hospital-acquired pneumonia) and focused on identification of genes relevant to antibiotic resistance.MethodsWhole genome analysis of A. baumannii DMS06669 from hospital-acquired pneumonia patients included de novo assembly; gene prediction; functional annotation to public databases; phylogenetics tree construction and antibiotics genes identification.ResultsAfter sequencing the A. baumannii DMS06669 genome and performing quality control, de novo genome assembly was carried out, producing 24 scaffolds. Public databases were used for gene prediction and functional annotation to construct a phylogenetic tree of the DMS06669 strain with 21 other A. baumannii strains. A total of 18 possible antibiotic resistance genes, conferring resistance to eight distinct classes of antibiotics, were identified. Eight of these genes have not previously been reported to occur in A. baumannii.ConclusionsOur results provide important information regarding mechanisms that may contribute to antibiotic resistance in the DMS06669 strain, and have implications for treatment of patients infected with A. baumannii.
Highlights
Acinetobacter baumannii is an important nosocomial pathogen that can develop multidrug resistance
The A. baumannii DMS06669 strain was resistant to all antibiotics tested with high level of minimum inhibitory concentration (MIC) values except colistin and tigecycline
And annotation of the genomic sequence Raw reads from the Illumina HiSeq mahcine were assessed with FastQC and filtered using Trimmomatic to MIC
Summary
Acinetobacter baumannii is an important nosocomial pathogen that can develop multidrug resistance. We characterized the genome of the A. baumannii strain DMS06669 (isolated from the sputum of a male patient with hospital-acquired pneumonia) and focused on identification of genes relevant to antibiotic resistance. The nosocomial pathogen Acinetobacter baumannii has become a serious world-wide health concern, as it has in recent decades acquired resistance to an array of antibiotics [1,2,3]. Antibiotic resistance of DMS06669 to multiple antibiotics was characterized using an antibiotic susceptibility assay test, and potential antibiotic resistance genes in DMS06669 were predicted from functional annotation analysis. Identification of these genes is an important step in
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