Abstract
Isolated and combined damaging effects of PbO and CuO nanoparticles have been estimated on an established line of human fibroblasts using three different cytotoxicity indices based on reduction in: (a) the cellular dehydrogenase activity (MTT Assay), (b) the ATP content (Luminescent Cell Viability Assay), (c) the cellular proliferation, viability, spreading, and attachment to substrate evaluated integrally by continuous impedance-based measurement of the Normalized Cell Index. For all these indicators a clear dependence of cell damage on concentration of metal oxide nanoparticles has been demonstrated for both types of metal oxide nanoparticles, which is adequately described by the hyperbolic function, while at the same level of exposure the quantitative characteristics of cytotoxicity of PbO-NPs in comparison with CuO-NPs are similar. The latter was previously observed in the subchronic experiment on rats. The combined in vitro cytotoxicity of nanoparticles has been also described mathematically using the response surface construction methodology and found to be ambiguous, which is also consistent with the conclusions from the experiment on rats with the same nanoparticles.
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