Abstract
A new technique has successfully established the non-obese diabetic/severely combined immunodeficiency (NOD/SCID) mouse model of ovarian cancer. Under 4% chloral hydrate (0.1 mL/g dose) anesthesia, female mice were inoculated with tumor-cell suspension. The expression rate of OVCAR3 to CA125 was assessed using flow cytometry. The inoculated site was hand palpated and the signs and symptoms related to tumor growth were observed with the naked eye. The allophycocyanin (APC) indirectly labeled mouse-antihuman CA125 and fluorescein isothiocyanate (FITC)-labeled anti-mouse MHC Class I molecule (H-2Kd/H-2Dd) were observed using a confocal laser scanning microscope. The animal model of ovarian cancer constructed using this method can more directly reflect the characteristics of cancer cells. It provides reliable experimental results and presents a technical platform for the research of ovarian cancer stem cells.
Highlights
There are several methods by which to construct an animal model of epithelial ovarian cancer: Nude Mice Model [1] of a subcutaneous transplanted tumor, Model [2] of an intraperitoneal transplanted tumor, Nude Mice Model [3] of an omentum transplanted tumor, and Model [4] of an orthotopic transplanted or metastatic tumor
The present orthotopic transplantation technique [5,6,7,8,9] is to first construct a Nude Mice Model of a subcutaneous transplanted tumor to supply the organism with cancer-cell strains, extract a small mass of the tumor tissue, open the ovarian capsule in the mouse under a dissecting microscope, and transplant the tissue mass into the ovarian parenchyma
1 microscopic neoplasia was observed in group A (1/10)
Summary
There are several methods by which to construct an animal model of epithelial ovarian cancer: Nude Mice Model [1] of a subcutaneous transplanted tumor, Model [2] of an intraperitoneal transplanted tumor, Nude Mice Model [3] of an omentum transplanted tumor, and Model [4] of an orthotopic transplanted or metastatic tumor. The present orthotopic transplantation technique [5,6,7,8,9] is to first construct a Nude Mice Model of a subcutaneous transplanted tumor to supply the organism with cancer-cell strains, extract a small mass of the tumor tissue, open the ovarian capsule in the mouse under a dissecting microscope, and transplant the tissue mass into the ovarian parenchyma. This technique can only indirectly reflect the characteristics of ovarian cancer-cell strains. It is a very difficult procedure and requires special equipment, such as a dissecting microscope; a new technique was used in our experiment: the cell
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