Abstract
The understanding of plant physiological complexity requires 3D imaging of the entire organ with subcellular resolution. However, most plant organs are highly opaque to light and their study under optical sectioning microscopes is difficult. By gathering the latest developments in the field of optical clearing of pigmented plant tissues, it was found that the clearing tannic roots (CTR) protocol drastically reduces light absorption by a depigmentation step and light scattering by the homogenisation of refractive indices through entire grapevine roots.
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