Abstract
[ 35 S] H 2 SO 4 was quantitatively reduced to [ 35 S] H 2 S with a pretreated mixture of HI/HCl/H 3 PO 2 . [ 35 S] H 2 S was used in a (S) homoserine sulfhydrylase [EC.4.2.99.10] catalysed thiol exchange reaction with O-acetyl-(S) homoserine to afford [ 35 S] (S) homocysteine. The latter was methylated (CH 3 I/NaOH) in situ giving rise to [ 35 S] (S) methionine which was purified by HPLC. Overall yield 10-15%; specific radioactivity : > 46 Tbq/mmol e.g. > 1243 Ci/mmol; chemical and radiochemical purities: > 98%
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