New cell wall glycopolymers of the representatives of the genus Kribbella

Abstract

Each of the cell walls of four representatives of the genus Kribbella (order Actinomycetales; suborder Propionibacterineae; family Nocardioidaceae) contains a neutral polysaccharide and an acidic polysaccharide with unusual structures. Common to all four strains studied is a mannan with the following repeating unit: ▪ In the cell wall of the strain VKM Ac-2541, a teichulosonic acid was identified with a monosaccharide component that has not hitherto been found in Gram-positive bacteria, viz., pseudaminic acid, and an unusual linkage type in the polymeric chain, ▪ where R = Н (45%), α- d-Gal p3OMe (37%) or α- d-Gal p2,3OMe (18%). The anionic cell wall components of three other strains are represented by teichuronic acids with a rare constituent, viz., a diaminosugar, 2,3-diacetamido-2,3-dideoxyglucopyranose. The structures of their repeating units differ in the nature of the acidic components: →4)-β- d-Man p2,3NAcA-(1→6)-α- d-Glc p2,3NAc-(1→ (VKM Ас-2538 and VKM Ас-2540) and →4)-β- d-Man pNAcA-(1→6)-α- d-Glc p2,3NAc-(1→ (VKM Ас-2539). The structures of all the glycopolymers were established by chemical and NMR spectroscopic methods; they are identified in Gram-positive bacteria for the first time.