New brucellosis erythrocyte diagnosticum for IHT in comparison with other serological methods (SAT, CFT, RBT and IDT)

Abstract

Relevance. Brucellosis is registered in many countries of the world, including Russia. The most unfavorable situation in the Russian Federation has developed in the Southern and North Caucasus Federal Districts. In the first place among the number of identified sick animals is the Dagestan Republic, where, despite the efforts of veterinary specialists, it is not possible to reduce the number of oci of brucellosis. With the help of classical methods (SAT, CFT), only 70% of animals infected with brucellosis can be detected. In this regard, domestic and foreign researchers have done a lot of work to improve existing and develop new diagnostic tools and methods.Methods. One of the most sensitive methods for the diagnosis of brucellosis is the indirect hemagglutination reaction (IHT). In comparative tests in IHT, with an improved antigen, good results were obtained.Results. The possibility of obtaining a highly active erythrocyte diagnosticum has been established with the combined use of two new surfactants: Progress products manufactured by AMC Media LLC (LosinoPetrovsk, Moscow region) and sodium dodecyl sulfate. The most rational parameters of the use of detergents for the treatment of brucella suspension and their optimal concentrations have been determined. It was found that IHT with a new antigen is specific and highly sensitive, compared with other serological reactions.