New Bioplastic Material Based on Hyaluronic Acid Hydrocolloid

Almazov Ia Rakhmatullin Rr

doi:10.4172/2155-9554.1000215

Abstract

Relief of the typical pathological processes and the early activation of regenerative mechanisms are the conditions for the successful wound healing. Reparative potential of epithelial tissues is realized at a balanced migration and proliferation of their cellular elements, synthesis of fibrous proteins and extracellular matrix components, epithelisation. An in-depth assessment was made of the structure of the developed bioplastic material based on hyaluronic acid hydrocolloid (G-Derm) and of its properties, positive in terms of optimizing the reparative mechanisms. Scanning probe microscopy and ultrastructure evaluation of bioplastic material were performed. For investigating the visualized globular structures, homogeneous samples of the material length 101.5 ± 11.2 nm, width 110.3 ± 10.7 nm, height 23.4 ± 3.4 nm were taken. The space between globular formations was 127.2 ± 21.3 nm. It was found that the roughness coefficient of the surface relief (Rq) was 8.7 ± 0.5 nm. The method of fixing the contact angle of water was used to evaluate hydrophilic/hydrophobic properties of biomaterial. Value of the angle was 83°; adhesion coefficient was 99.88 mN/ m2, which characterizes the surface of biomaterial as moderately hydrophilic. Mesenchymal stromal stem cells were cultured on a substrate of bioplastic material based on hyaluronic acid hydrocolloid. In the course of ultrastructural study cell migration into the material was recorded, presence between the interwoven fibrillar fibres of cell layers of an oblong shape 3.7 ± 0.5 mcm wide, which reflects their cooperation with the surface of the developed polymer suitable for the use as a growth substrate in case of biotechnological replacement of tissue defects.

Highlights

Relief of typical pathological processes and early activation of regenerative mechanisms are the conditions for successful healing of defects in epithelial tissues of various etiologies
It has been ascertained that histoequivalent-bioplastic material based on hyaluronic acid hydrocolloid visualized by scanning atomic force microscopy (AFM) has a morphologically homogeneous surface, the surface ultrastructure being represented by globular formations of the same type (Figure 1)
In the course of morphometry it was determined that the dimensional parameters of the visualized globular structures of bioplastic material based on hyaluronic acid hydrocolloid were as follows: width 110.3 ± 10.7 nm, height 23.4 ± 3.4 nm

Summary

Introduction

Relief of typical pathological processes (microcirculatory disorders, interstitial oedema, uncontrolled inflammatory reaction, respiratory burst, acidosis, hypercytokinemia) and early activation of regenerative mechanisms are the conditions for successful healing of defects in epithelial tissues of various etiologies. Reparative potential of epithelial tissues is realized at a balanced migration and proliferation of their cellular elements, synthesis of fibrous proteins and extracellular matrix components, epithelisation [1]. For the purpose of plastic reconstruction of epithelial tissue defects as well as for ensuring self-healing of wounds and burns, various bioplastic materials based on synthetic and natural (preferably) polymers are extensively used [2,3]. Determination of the optimal physicochemical, mechanical and biological properties of natural bioplastic materials used for replacement of the epithelial tissue defects is extremely important and relevant in particular in terms of the ability of these polymers to ensure the optimal conditions for skin cells adhesion, migration and proliferation [4,5]. The presence of the surface potentials, specific features of the surface structure as well as its composition are the necessary characteristic which can affect the viability and proliferation of cells at interaction with bioplastic materials [7,8,9]

Methods

Results

Conclusion