New automation-friendly 96-well plate ready-to-use 8-color dry antibody panel for basic phenotyping of human whole blood and peripheral blood mononuclear cells using flow cytometry

Abstract

Abstract Automation-friendly 96-well plate flow cytometry solutions are ideal for pharmaceutical, academic, and CRO laboratories that perform testing on large numbers of samples and/or large number of test conditions. Our ready-to-use pre-formulated dry antibody panel in 96-well plate format provide researchers all the reagents within the wells of the plate where the test is conducted in a stable dry format, resulting in dependable reagent performance and reduction of manual pipetting steps with the added benefit of time and material (reagent and sample) savings. In addition, the 96-well plate format is compatible with automated liquid handlers for walk-away flexibility and higher-throughput sample processing. Here we show flow cytometry data for both whole blood and PBMC samples stained with the first ready-to-use DURAClone IM Phenotyping BASIC plate (RUO) in a shallow 96-well plate containing a dry reagent antibody panel to identify major lymphocyte and monocyte subpopulations for the following markers: CD45, CD16, CD56, CD19, CD14, CD3, CD4 and CD8. Processing of whole blood samples using the shallow 96-well plate format was achieved using FLEXLyse, a lysing solution with low volume ratio of lysis/whole blood, applicable for no-wash and wash protocols. Our experiments were performed using automation on the Biomek i7 liquid handling automated workstation integrated with a CytoFLEX LX flow cytometer and microplate centrifuge demonstrating a fully automated flow cytometry workflow.