Abstract
Fusarium oxysporum is an important pathogen that can reduce corm and flower production of Gladiolus. Cell suspensions of four Fusarium susceptible Gladiolus cultivars (Friendship, Peter Pears, Victor Borge and Novalux) were found highly sensitive to the fusaric acid (FA). Gradual increase in FA concentrations to the cell-suspension cultures decreased cell growth considerably. Plantlets of all the selected cell lines showed significant resistance as compared with control in the presence of 0.5 mM FA. The in vitro selected cell line numbers CAMB-G01, CAMB-G04, CAMB-G06 and CAMB-G09 did not affect at all and showed an Average Severity Index of zero as compared with the control of the Friendship cultivar. The cormlets of all in vitro selected cell lines of Friendship were inoculated with a conidial suspension of Fusarium oxysporum before planting and also sprayed with the same spore suspension when the height of plants was about 6 cm for further characterization. The four selected cell lines showed the same response whether or not inoculated with conidia of Fusarium oxysporum. Plantlets of all the selected cell lines exhibited significant growth as compared with control after application of conidia of Fusarium oxysporum.
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