New Anti-inflammatory Flavonol Glycosides from Lindera akoensis Hayata.

Chung-Ping Yang,Shih-Chang Chien,Pei-Hsin Shie,Guan-Jhong Huang,Yueh-Hsiung Kuo

doi:10.3390/molecules24030563

Abstract

Inflammation is related to many diseases. Lindera akoensis Hayata was often used in folk therapy in Taiwan for inflammation. In this study, three new flavonol acyl glycosides, namely kaempferol-3-O-β-D-4′′,6′′-di-(E)-p-coumaroylglucoside (1), 3′′-(E)-p-coumaroylafzelin (2) and 4′-O- methyl-2′′,4′′-di-(E)-p-coumaroylquercitrin (3), and three components, 3β-dodecyl-4β-hydroxy- 5β-methyldihydrofuran-2-one (4), 2β-acetoxyclovan-9α-ol (5), (1α,4β,6β)-trihydroxyeudesmane (6) that were isolated from the natural product for the first time were obtained along with 25 known compounds from L. akoensis. Their structures were determined by comprehensive spectroscopic analyses (1D and 2D NMR, EI-, ESI- and HRESI-MS). The ability of 1 to decrease the LPS-stimulated production of nitrite in RAW264.7 cell was evaluated, showing an IC50 value of 36.3 ± 3.2 μM. This result supports the value of L. akoensis as a traditional medicine resource.

Highlights

Lindera akoensis Hayata belonging to the Lauraceae family, it is an endemic species widely distributed in central and southern Taiwan
40 -O-methyl-200,400 -di-(E)-p-coumaroylquercitrin (3), and three compounds that were isolated from the natural product for the first time, 3β-dodecyl-4β-hydroxy-5β-methyldihydrofuran-2-one (4), 2β-acetoxyclovan-9α-ol (5), and (1α,4β,6β)-trihydroxyeudesmane (6) (Figure 1) along with 25 known compounds, including two monoterpenoids, 17 sesquiterpenoids, and six steroids
L. akoensis is only used for ornamental purposes and some inflammation treatments

Summary

Introduction

Lindera akoensis Hayata belonging to the Lauraceae family, it is an endemic species widely distributed in central and southern Taiwan. It has been used by local residents to treat various inflammation symptom [1]. In order to confirm the traditional folk usage of L. akoensis, this study continued our previous work on the isolation and purification of L. akoensis components. Six novel compounds (Figure 1) and 25 known compounds were isolated and identified. Based on our experimental results, compound 1 has anti-inflammatory activity by decreasing nitric oxide (NO)

Methods

Results

Conclusion