Abstract
The objective of this study was to elucidate the antibacterial effect of hypertonic saline of KH2PO4 and NaCl solutions for enhancing hospital hygiene facing drug resistance of the biofilm of Pseudomonas aeruginosa isolated from a hospital environment on an agar cetrimide medium. The variations of P. aeruginosa biofilm density were measured following optical surface density by a correlated imaging method. Inhibition tests of 90% of the biofilm in a selective liquid medium monitored by spectrophotometry showed inhibition rates of 91.2 and 91.1% for minimal biofilm inhibition concentrations of 5.4 and 3.6% for NaCl and KH2PO4, respectively. The results of the eradication tests of 50% of the biofilm showed minimal biofilm eradication concentrations of 6.3 and 3.6% and eradication ratios of 50.6 and 50.9% for NaCl and KH2PO4, respectively. In the case of a selective solid medium, the results exhibited a minimal biofilm inhibition concentration of 4.2 and 3.9%, a biofilm inhibition rate of 90.8 and 90.5%, and ...
Highlights
Pseudomonas aeruginosa, with Staphylococcus aureus, is one of the most common bacteria causing respiratory and nosocomial infections
The objective of this study was to elucidate the antibacterial effect of hypertonic saline of KH2PO4 and NaCl solutions for enhancing hospital hygiene facing drug resistance of the biofilm of Pseudomonas aeruginosa isolated from a hospital environment on an agar cetrimide medium
Inhibition tests of 90% of the biofilm in a selective liquid medium monitored by spectrophotometry showed inhibition rates of 91.2 and 91.1% for minimal biofilm inhibition concentrations of 5.4 and 3.6% for NaCl and KH2PO4, respectively
Summary
Pseudomonas aeruginosa, with Staphylococcus aureus, is one of the most common bacteria causing respiratory and nosocomial infections. In European intensive care units, the prevalence of these bacteria is approximately 4%, and its attributable mortality is around 13.5%, even with adequate antibiotic treatment.[1] In the multidrug resistance strains, the mortality rises up to 35.8% and the presence of multidrug resistance strains is identified as an independent predictor of hospital death.[2,3] In case of attack, the organism can be involved in the respiratory urinary, wound, and blood stream infections, mainly in patients with severe underlying diseases or impaired immune defense.[4]. P. aeruginosa resistant to diverse antibiotics appears as a potentially dangerous bacterium for the patients suffering from cystic fibrosis and comorbidities and in immunosuppressed patients and acquired immune deficiency syndrome sufferers. Those admitted in intensive care or the cancer patients for Received: October 9, 2018 Accepted: December 25, 2018 Published: January 8, 2019
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