Abstract
Simultaneous measurement of neutrophil migration, phagocytic activity, candidacidal and bactericidal activity were made during quadruple chemotherapy of advanced Hodgkin's disease (HD). Measurements were also made in normal individuals, hospital patients not on chemotherapy, untreated patients with advanced HD and patients off chemotherapy for over a year. Neutrophil migratory activity was usually normal in untreated HD patients and those on chemotherapy, but less than 20% of all tests showed depressed values, some of which were corrected by plasma. Similar results were found with neutrophil phagocytosis. Abnormalities in these functions were found in both early and late cycles, but there was a tendency for migration to deteriorate during later chemotherapy cycles. Neutrophil candidacidal and bactericidal activity were frequently depressed in patients on treatment and there was deterioration in candidacidal activity during the chemotherapy cycle. These abnormalities of killing activity were frequently corrected in control plasma. Neutrophil function is normal in most patients with advanced HD and in patients in remission. In a minority of patients on treatment there are marked functional defects, especially in killing activity. These defects are partly cell-associated and partly plasma-related. Susceptibility to infection during chemotherapy of HD may be partly due to defective neutrophil function.
Highlights
Summary.-Simultaneous measurement of neutrophil migration, phagocytic activity, candidacidal and bactericidal activity were made during quadruple chemotherapy of advanced Hodgkin's disease (HD)
Neutrophil function has been the subject of some investigation, but reported studies have usually concerned themselves with single aspects of neutrophil function, and have not always distinguished between the effects of disease and of treatment
We have studied several white-cell functions simultaneously in patients with advanced HD over many cycles of combination chemotherapy
Summary
To combine measurement of phagocytosis and Preparation of leucocytes.-50 ml of blood killing in the same test we chose a 2:1 ratio. The number of dead suspension was used in all tests of neutrophil Candida per cell was called the killing index. 79-90o neutrophils, 12*6° lymphocytes, 7-5o frequently >2 because the whole cell susmonocytes for both controls and patients. All function tests were carried out in both because some degree of Candida multiplicaautologous and control plasma. Plasma was obtained from the normal control phagoeytosis is better assessed by subject who was tested with each batch of an assay which measures rate of ingestion, samples. Tests wAere carried out in duplicate, patients had low cell counts, it was not poswith control or autologous plasma, and 00 kill sible to do all tests on every occasion. When the data is transformed into square roots and analysed, the tests for statistical significance give identical results
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