Abstract
Zymogen-stimulated neutrophils release many pre- and non-preformed factors including proteolytic enzymes, oxygen free radicals and arachidonic acid metabolites. Measurement of the histamine concentration of a supernatant fluid commonly employs an organic extraction procedure followed by condensation to o-phthalaldehyde. Diamine oxidase digestion studies and high-performance liquid chromatographic analysis suggest that a factor(s) derived from zymogen-stimulated neutrophils can produce a positive interference in the fluorometric determination of histamine.
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