NEUTROPHIL AND LYMPHOCYTES OF RAT PUPS OF THE DOES EXPOSED TO CIGARETTE SMOKE TREATED WITH ETHANOL EXTRACT OF KEBAR GRASS (Biophytum petersianum Klotzsch)

Abstract

Kebar grass contains vitamin A and vitamin E, which are antioxidant compounds that are thought to improve the blood picture of rats that are disturbed due to the exposure of the free radicals from cigarette smoke. The purpose of this study was to determine the neutrophil and lymphocyte profiles of rat pups of the does which treated with the ethanol extract of kebar grass (Bhiophitum petersianum Klotzsch) due to the exposure toward the cigarette smoke. This study used an experimental method by using a Completely Randomized Design (CRD) which consisted of 4 (four) treatment groups and 3 (three) replications. After the treatment, all groups of does were mated and the pups that were born would be taken 3 in each group as a replication, and then their blood would be taken to analyze the neutrophils and lymphocytes. The data which successfully obtained were analyzed by analysis of variance (ANOVA). The results showed that there was a change in the neutrophil and lymphocyte profiles of rat pups from does which were treated with ethanol extract of kebar grass due to the exposure toward cigarette smoke, in which there was an increase in the percentage of neutrophils and lymphocytes (P<0.05), where an increase in the percentage of neutrophils to normal direction while the increase in the percentage of lymphocytes occurred continuously. The increase in lymphocytes is strongly suspected to be due to the administration of ethanol extract of kebar grass which contains flavonoids, resulting in the proliferation of lymphocytes as antibody products.