NEUTROPHIL ACTIVATION DURING HEMORRHAGIC SHOCK AND RESUSCITATION IN RABBITS

Abstract

S137 Introduction: Hemorrhagic shock and resuscitation represents generalized form of ischemia-reperfusion phenomenon. Previous investigations indicate neutrophils are activated during ischemia-reperfusion and these activated neutrophils play an important role in tissue injury [1]. Also several reports revealed that anesthetic agents differently influenced neutrophil activity [2,3]. The purpose of this study was to compare the effect of isoflurane and propofol on neutrophil activation during hemorrhagic shock and its resuscitation. Methods: With IRB approval, 20 rabbits were anesthetized either with isoflurane (n=10) or propofol (n=10). Mean arterial pressure (MAP) was maintained below 40mmHg for 60 min to simulate hemorrhagic shock state. There after shed blood was retransfused to maintain MAP above 70mmHg. Blood gas and hematological analysis were performed at 0(preshock), 60 (end of shock), 120 and 180 min. Neutrophil activation was analyzed by luminol-dependent chemiluminescence(CL) at the same time period. These data were statistically analyzed by unpaired t-test and ANOVA when appropriate, p<0.05 considered significant. Results: MAP, heart rate, base excess and plasma lactate did not differ between isoflurane and propofol during study period. Thus hemodynamics and tissue perfusion during and after hemorrhagic shock seemed identical in both groups. However, CL was significantly increased after resuscitation in isoflurane group compared to propofol group (Figure 1).Figure 1Discussion: Neutrophils were significantly activated during resuscitation in isoflurane group compared to propofol group. Since neutrophil activation is involved in the pathophysiology of posttraumatic complications [4], propofol may be advantageous in anesthetic management of hemorrhagic shock.