Abstract

Illustrative neutron scattering studies are presented of rotational modes of bound H 2O in live leaves of Ficus elastica decora, Philodendron cordatum and Peperomia obtusifolia. Interpretation of data is based on the hypothesis that hindrance of rotational motion should result in shifts to higher frequency. Such shifts are verified by comparing scattering from water with that from ice and cytosine monohydrate. Statistical analysis of the scattering from plant leaves is based on the assumption that least-squares fitting can be carried out using variable percentages of ice and water spectra to describe hydrate and free water fractions. It is found that the amount of water present as a 4-fold bonded hydrate is very small, being of the order of a few percent or less.

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