Neutralizing Effect of Solanum dasyphyllum Schumach. &Thonn Extract against Naja nigricollis Venom-induced Toxicity

Abstract

Background: Clinical treatments for snakebite envenomation typically involve the administration of antivenom, which can lead to various side effects. However, due to the limitations of conventional antivenoms in effectively treating snakebite envenomation, scientists are now exploring alternative sources for potential antivenom compounds, particularly those derived from plants. Solanum dasyphyllum belongs to the Solanaceae family. This plant has been found to possess several beneficial properties, including neuromuscular, anti-poisoning, and antispasmodic effects. Aim of the Study: This study aimed to investigate the phytochemical constituents and the In-vivo detoxifying effect of S. dasyphyllum extract against N. nigricollis-induced toxicity. Materials and Methods: Phytochemical screening and anticoagulant assays were conducted using standard procedures. The neutralizing and detoxifying effects of S. dasyphyllum were investigated in-vivo using locally bred adult Swiss mice. The neutralizing effect was evaluated in mice by administering a mixture of N. nigricollis venom and methanol leaf extract of S. dasyphyllum (100-400 mg/kg). The detoxifying effect was investigated by administering S. dasyphyllum via the intramuscular route 10 minutes after inoculation with N. nigricollis venom through the intraperitoneal route. Results: The results of phytochemical screening revealed the presence of tannins, cardiac glycosides, saponins, flavonoids, and alkaloids, and the absence of anthraquinone and starch. The results of the clotting time of human blood treated with N. nigricollis venom showed a significant reduction in the clotting time with an increase in the concentration of methanol leaf extract of S. dasyphyllum. Additionally, the mortality of the mice that were pre-treated with N. nigricollis venom before injected with various doses of plant extract and those that were co-administered with the venom and plant extract significantly lower compared to mice administered with the venom alone. S. dasyphyllum significantly reduced the extent of lipid peroxidation and the activity of the enzyme acetylcholinesterase compared to the untreated group. Conclusions: S. dasyphyllum possesses antivenom activity against N. nigricollis, and this result further supports the ethnomedical use of the plant in the treatment of snakebite envenomation.