Abstract
Amino acids that are ampholytes can be effectively separated and purified by the method of neutralization dialysis (ND), whose advantage is the ability to control the pH value of the solution without adding reagents. An important task is to optimize the parameters of the ND process to ensure minimal losses of amino acids during their isolation from mixed solutions. An experimental study of the process of demineralization of the phenylalanine and sodium chloride equimolar mixture by the ND method was carried out. It is established that varying the concentration and flow rate of acid and alkali solutions in the corresponding compartments of the dialysis cell allows for regulating the pH value of the solution being desalted and controlling the amount of amino acid loss. Halving the acid concentration (from 0.10 to 0.05 M) allowed reducing the losses of phenylalanine from 18.3 to 16.4%, and using a lower solution flow rate in the acid compartment (0.75 instead of 1.50 cm s–1) made it possible to reduce these losses to 14.2%. At the same time, in all experiments, the electrical conductivity of the desalted solution decreased by 90%, which suggests a high degree of demineralization and the effectiveness of the method used to isolate phenylalanine from the mixed solution.
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