Neurotransmitters Regulating Vestibular Commissural Inhibition in the Cat

Abstract

The present study focuses on the transmitters which control the vestibular neural activity and, in particular, those which regulate commissural inhibition. Extracellular spike recordings were taken from single vestibular neurons of decerebrate cats. Seven-barreled electrodes were used. The barrels, except for the central one, were filled with several transmitter candidates and their specific inhibitors. After isolation of a type I neuron, the chemicals were iontophoretically applied to examine their effects on the activity of the neuron. The results were as follows: i) GABA and glycine markedly decreased spontaneous firing of the neurons, while serotonin did not change the activity. ii) Bicuculline abolished the inhibitory effects of GABA on the neurons. iii) Strychnine also abolished the effects of glycine, iv) Commissural inhibition caused by electrical stimulation of the contralateral labyrinth was not abolished by the application of strychnine but was abolished by bicuculline. We concluded that 1) vestibular type 1 neurons are controlled by GABAergic and glycinergic neurons, and that 2) the commissural inhibition is activated by the GABAA receptor, but not by the GABAB receptor.