Abstract
A 3.0 mg dose of physostigmine or normal saline was given intravenously to 23 normal subjects who had been treated with probenecid. Homovanillic acid and 3-methoxy-4-hydroxyphenylglycol concentrations were significantly higher in the lumbar cerebrospinal fluid of subjects who received physostigmine than subjects who received normal saline. This establishes biochemical evidence for a cholinergic link in the central nervous system of man.
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