Neurotoxicity of interleukine-1 beta on hippocampus neuron in vitro

Abstract

Objective To examine cell viability and brain-derived neurotrophic factor( BDNF) concentration in the effect of interleukine-1 β(3 (IL-1β) on hippocampus neuron primary cell culture. Methods Hippocampus were dissected and cultured from new-born SD rats,the cultured cells were fixed on 7 days and immunocyto-chemically stained with neuronal specific enolase (NSE) antibody. Interleukine-1 beta IL-1β 0ng,5ng/ml, 10ng/ ml,20ng /ml was applied to no serum cultured neurons for 48 h. The cell proliferation and viability was determined by methyl thiagolyl tetragolium( MTT) assay, BDNF concentrations in the supernatant was determined by ELISA kits. Results Immunocytochemically stained with NSE antibody results indicated that above 90% of cell isolated from hippocampus of 7 d rats greed well and expressed NSE immunoreativity. The cell viability decreased follow with the dose of IL-1β, the optical density of each dose detected at 590 nm wave were(0.9462 ± 0.2972;0.5585 ± 0.2407; 0.4295 ±0.1401;0.4191 ±0.1050), compared with normal group ( P<0.05, P<0.01 , P<0.01). BDNF concentrations in the supernatant was decreased compared to control group,and the contents of BDNF in each group were[(12.4±1.77)pg/ml; (8.65±0.71)pg/ml;(8.9±0.35)pg/ml;(7.9±0.35)pg/ml], compared with normal group ( P<0.05, P<0.05, P<0.05). Conclusion Neurotoxicity of IL-1β on hippocampus neuron in vitro might via decreased synthesis and secretion of BDNF. Key words: Interleukine-1β; Neuron; BDNF; Hippocampus; MTT assay