Abstract
Effects of neurotensin on the release of endegenous noradrenaline (NA) from rat hypothalamic slices were investigated. NA was extracted by the aluminum adsorption method, and assayed electrochemically by high-performance liquid chromatography. Neurotensin (30–300 μM) enhanced the release of NA, in a dose dependent manner. Neurotensin 2 and 10 μM, doses without effect on the spontaneous release of NA, potentiated the potassium-induced release of this amine. Neurotensin 100 μM repetitively added to the medium at 40 min intervals induced a consistent and reproducible increase in the release of NA. This neurotensin-induced release of NA was completely abolished in calcium free medium containing EGTA 1 mM. The presence of tetrodotoxin 2 μM did not alter the effect of neurotensin 100 μM. These results of in vitro investigations suggest that neurotensin has a direct effect on NA nerve terminals in the hypothalamus and releases NA, in a Ca ++-dependent manner.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
