Abstract
The specific activity of Neurospora crassa glutamine synthetase varies according to the nitrogen source in which the organism is grown. In a poor nitrogen source such as glutamate, the specific activity of the enzyme is higher than that found in good nitrogen sources such as ammonium or glutamine. These differences in specific enzyme activity correspond to differences in enzyme concentration. The relative rates of glutamine synthetase synthesis and degradation were measured in exponential cultures grown in different nitrogen sources. The differences in enzyme concentration are explained by differences in the relative rate of enzyme synthesis.
Highlights
The specific activity of Neurospora crassa glutamine synthetase varies according to the nitrogen source in which the organism is grown
(161, we have developed a sensitive assay to measure the relative rates of enzyme synthesis and degradation
Data presented in this study indicate that the differences in specific glutamine synthetase activity found in N. crussa grown
Summary
From the Departamento de Biologia Molecular, Instituto de Investigaciones National Autdnoma de Mkxico, Mkxico 20, D. The relative rates of glutamine synthetase synthesis and degradation were measured in exponential cultures grown in different nitrogen sources. In studies in which organisms are grown in well defined media, it has been found that the enzyme-specific activity varies according to the nitrogen sources present (l-7). The specific enzyme activity is high when the organism is grown in glutamate as the sole nitrogen source and low when ammonium or glutamine are present in the culture medium. These differences in specific enzyme activity have been found to correlate with differences in enzyme concentration.’. Exponentially in different nitrogen sources are due to differences in the relative rate of enzyme synthesis
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
