Neurosecretion in Experimental Condition. III. Phase Contrast Microscopic Observations on the Hypothalamo-hypophysial Neurosecretory System of the Experimentally Dehydrated Dog

Abstract

The hypothalamic neurosecretory cell and posterior pituitary of dog in experimentally dehydrated condition were observed under phase contrast microscope and, with non-fixed and fixed sections, following results were obtained.1. Under dehydrated condition, neurosecretory granules observed by phase contrast microscopy showed a heavy decrease in both hypothalamic neurosecretory cells and the posterior lobe. This is in accordance with findings of gomoriphil granules in GOMORI's staining.2. NISSL's substance of hypothalamic neurosecretory cells under dehydrated condition shows also by phase contrast method findings of hyper activity.3. That under dehydrated condition the findings of the granules observed by phase contrast microscopy and those of gomoriphil granules are in accordance with each other is a basis on which the granules observed by phase contrast microscope are to be identified with neurosecretory granules.4. No cell division was observed in pituicytes of 6 cases of dehydrated dogs.