Abstract
1. A standard assay for the content of light-adapting distal retinal pigment hormone in crustacean eyestalk extracts is described. Linear regression equations for the relation between response of the retinal effectors of test Palaemonetes and concentration of eyestalk extract from Palaemonetes and from Libinia have been calculated.2. A Palaemonetes unit of this hormone is defined as that concentration of eyestalk extract, injected into a minimum of 10 dark-adapted P. vulgaris measuring 35—40 mm. in rostrum-telson length, which will result in an average distal retinal pigment index of 0.150. For the Palaemonetes and Libinia used in this study 1 Palaemonetes unit is equivalent respectively to concentrations of 3.5 and 0.34 eyestalks per 1.0 ml.3. Thermostability, small molecular size, complete or partial inactivation by tissue extracts (peptidases?) and by crystalline trypsin and chymotrypsin are properties of the hormone consistent with a possible peptide structure.
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