Neuroprotector effects of Magnesium Sulfate (MgSO4) used in Piglets after Hypoxic-Ischemic Insult † 1903

Abstract

Magnesium sulfate has been proposed as a potential strategy for hypoxic-ischemic insult because its ability to block calcium entry through NMDA receptor. Objetive: the aim of this work was to examine the neuroprotector effect of MgSO4 in an experimental piglet model of hypoxic-ischemic insult when is administrated as a rescue strategy, 15 minutes after the insult. Methods: Piglets (5 days old) were assigned to (S) Sham (n=8), (SM) Sham+MgSO4 (n=8), (HI) hypoxic-ischemic (n=8), (HIM) hypoxic-ischemic+MgSO4 (n=8) group. In the groups HI and HIM temporary occlusion of carotid arteries during 30 minutes (FiO2 8%), and hemorragic hypotension (15 min) after 15 min of hypoxic insult was made. A loading dosis of 300 mg/kg of MgSO4 followed by 150 mg/kg/h iv were administrated during 12 h to SM and HIM groups. CSF neuron specific enolase(NSE) levels were measured by EIA. Cerebral neuropathologic examination was performed and graded following a score; neuronal necrosis was assesed in diferents regions of the brain. Results: Significant increase in CSF NSE levels in the inmediately before sacrifice were found in the HI and HIM groups (p<0.05). Severe brain damage was found in groups HI and HIM in all of the sections examined, mainly in cortex, basal ganglia and hippocampus.Conclusion: We did not find neuroprotector effect when MgSO4 was administrated 15 minutes after the hypoxic-ischemic insult in this experimental model.