Abstract
Pressurized liquid extraction (PLE) conditions were optimized to improve the recovery of orange (Citrus sinensis) by-products terpenoids. The neuroprotective potential of the PLE extracts were tested against a set of in-vitro assay (antioxidant (ABTS), reactive oxygen/nitrogen species (ROS/RNS)) as well as enzymatic tests (acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and lipoxygenase (LOX)). Gas chromatography coupled to high-resolution mass spectrometry (GC-q-TOF-MS) analysis revealed a higher enrichment in mono- and sesquiterpenoids of the PLE extracts with the highest neuroprotection capacity. In-silico molecular docking analysis showed the specific interaction of representative terpenoids with enzymes active sites. The results demonstrate that the selected extract at 100°C and 30 minutes possesses high antioxidant (ABTSIC50=13.5μgmL-1; ROSIC50 = 4.4μgmL-1), anti-cholinesterase (AChEIC50=137.1 vg L-1; BChEIC50=147.0μgmL-1) and anti-inflammatory properties (against IL-6 and LOXIC50=76.1μgmL-1), with low cytotoxicity and protection against L-glutamic acid in cell models.
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