Neuroprotective effects of Liriope platyphylla extract against hydrogen peroxide-induced cytotoxicity in human neuroblastoma SH-SY5Y cells.

Hee Ra Park,Heeeun Lee,Won-Kyung Cho,Jong Wook Jeon,Hwayong Park,Jin Yeul Ma

doi:10.1186/s12906-015-0679-3

Abstract

BackgroundOxidative stress is involved in neuronal cell death and mitochondrial dysfunction in neurodegenerative diseases. Liriope platyphylla (LP) has been suggested to have anti-inflammation, anti-bacterial, and anti-cancer effects. However, whether LP exerts neuroprotective effects on neuronal cells is unknown.MethodsThe present study was performed to investigate the neuroprotective effects of LP extract (LPE) against hydrogen peroxide (H2O2)-induced injury in human neuroblastoma cells SH-SY5Y. To test neuroprotective effects of LPE, we performed cell viability assay, flow cytometry analysis and western blot analysis. In addition, mitochondrial membrane potential (MMP) and oxidative stress were performed to evaluate the anti-apoptotic and anti-oxidant effects.ResultsLPE pretreatment conferred significant protection against the H2O2-induced decrease of SH-SY5Y cell viability. H2O2-induced increases of intracellular oxidative stress and mitochondrial dysfunction were attenuated by LPE pretreatment. Therefore, LPE pretreatment prevented SH-SY5Y cell injury. Treatment with H2O2 significantly induced poly(ADP ribose) polymerase (PARP) and caspase-3 cleavage, which was blocked by LPE. We found that p38 activation was involved in the neuroprotective effects of LPE.ConclusionsCurrent findings suggest that LPE exerts neuroprotective effects against H2O2-induced apoptotic cell death by modulating p38 activation in SH-SY5Y cells. Therefore, LPE has potential anti-apoptotic effects that may be neuroprotective in neurodegenerative diseases and aging-related dementia.

Highlights

Oxidative stress is involved in neuronal cell death and mitochondrial dysfunction in neurodegenerative diseases
Effects of LP extract (LPE) on Hydrogen peroxide (H2O2)-induced cell loss in SH-SY5Y cells To determine the effects of LPE treatment on cell viability, SH-SY5Y cells were seeded in 96-well plates (5 × 104 cells/ml), cultured for 24 h, and treated with LPE at concentrations ranging from 0.05 to 500 μg/ml for 24 h
Cell Counting Kit-8 (CCK) analysis showed that H2O2 decreased SH-SY5Y cell viability, while 50 μg/ml LPE pretreatment conferred significant protection against H2O2-induced cell loss (Fig. 1b)

Summary

Introduction

Oxidative stress is involved in neuronal cell death and mitochondrial dysfunction in neurodegenerative diseases. Liriope platyphylla (LP) has been suggested to have anti-inflammation, anti-bacterial, and anti-cancer effects. Oxidative stress is involved in neuronal cell death, which is one of the major causes of neurodegenerative diseases, including Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis [1,2,3,4]. ROS are generally found in the course of apoptotic cell death caused by intracellular microenvironmental changes. In this context, inhibiting ROS generation can be a useful way to protect normal neuronal cells from. LP and red LP extract were reported to decrease amyloid-beta (Aβ1–42) peptide levels in the brain and increase nerve growth factor (NGF) levels in the serum of NSE/hAPPswe transgenic mice and Tg2576

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