Abstract
BackgroundRecent studies shows that hyperbaric oxygen (HBO) therapy exerts some protective effects against neural injuries. The purpose of this study was to determine the neuroprotective effects of HBO following sciatic nerve transection (SNT).MethodsRats were randomly divided into five groups (n = 14 per group): Sham-operated (SH) group, SH + HBO group, SNT group, and SNT + pre- and SNT + post-HBO groups (100% oxygen at 2.0 atm absolute, 60 min/day for five consecutive days beginning on 1 day before and immediately after nerve transaction, respectively). Spinal cord segments of the sciatic nerve and related dorsal root ganglions (DRGs) were removed 4 weeks after nerve transection for biochemical assessment of malodialdehyde (MDA) levels in spinal cord, biochemical assessment of superoxide dismutase (SOD) and catalse (CAT) activities in spinal cord, immunohistochemistry of caspase-3, cyclooxigenase-2 (COX-2), S100beta (S100ß), and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) in spinal cord and DRG.ResultsThe results revealed that MDA levels were significantly decreased in the SNT + pre-HBO group, while SOD and CAT activities were significantly increased in SNT + pre- and SNT + post-HBO treated rats. Attenuated caspase-3 and COX-2 expression, and TUNEL reaction could be significantly detected in the HBO-treated rats after nerve transection. Also, HBO significantly increased S100ß expression.ConclusionsBased on these results, we can conclude that pre- and post-HBO therapy had neuroprotective effects against sciatic nerve transection-induced degeneration.
Highlights
Recent studies shows that hyperbaric oxygen (HBO) therapy exerts some protective effects against neural injuries
Sciatic nerve transection in the sciatic nerve transection (SNT) group produced a significant elevation (p < 0.01) in lipid peroxidation level compared to SH and SH + HBO groups
Treatment with HBO in the SNT + post-HBO group did not produced a significant decrease in MDA levels compared to SNT group (p > 0.05)
Summary
Recent studies shows that hyperbaric oxygen (HBO) therapy exerts some protective effects against neural injuries. It has been postulated that the use of Hyperbaric oxygen (HBO) therapy, treatment providing 100% oxygen at a pressure greater than that at sea level, can be considered as one of these methods. In this regard, studies documented that HBO have neuroprotective effects against traumatic brain and spinal cord injury [11, 12], ischemic brain and spinal cord injury [13, 14], neurodegenerative disorders [15, 16], oxidative damage. We investigated the beneficial effects of HBO therapy on neuronal cell preservation after sciatic nerve transection
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