Neuroprotective Effect of Fagopyrum dibotrys Extract against Alzheimer's Disease.

Chen Liang,Jian-Ping Yuan,Yue-Qin Zeng,Tao Ding,Lv Yan,Lu Ling,Xin-Fu Zhou

doi:10.1155/2017/3294586

Abstract

Accumulated evidence suggests that polyphenolic antioxidants present in herbs play important roles in prevention of AD; the molecular mechanisms behind neuroprotective actions rely on the phenols through different effects on the amyloid-aggregation pathway. Fagopyrum dibotrys is a traditional herbal medicine which contains high quantity phenols. In present study, we investigate the beneficial pharmacological actions of Fagopyrum dibotrys extract in the APP/PS1 transgenic mouse mode; meanwhile, effects of the FDE on the fibrillation and cytotoxicity of Aβ peptide were evaluated in vitro. After 9-month treatment, FDE exhibited multifunctional properties on Aβ-related pathologies, which cleaned Aβ deposits in the brain and decreased Aβ burden in the plasma, inhibited microhaemorrhage, and reduced reactive microglia in APP/PS1 transgenic mice and also promoted Aβ fibrils disaggregation and inhibited neurotoxicity induced by Aβ in SH-SY5Y cells. These results highlighted that FDE is an AD type pathology modulator with therapeutic potential against AD.

Highlights

Alzheimer disease (AD) is progressive and neurodegenerative disorder disease among the elderly, characterized by progressive loss of memory and cognition [1]
To investigate the effects of Fagopyrum dibotrys extract (FDE) on the fibrillation and cytotoxicity of Amyloid β (Aβ) protein, the inhibitory effects of FDE on Aβ1–42 fibril formation were determined by using thioflavin T fluorescence (Th-T) and Electron Microscopy (EM); the protective effects against cytotoxicity induced by Aβ1–42 in SH-SY5Y cells were evaluated by MTT assay
The data from Th-T fluorescence assay showed that FDE had a dose-dependent effect on disaggregation of preformed Aβ1–42 fibril (ANOVA, F = 10.762, p < 0.001, Figure 2(c)), it reduced the neurotoxicity of Aβ1–42 on the cultured SH-SY5Y cells (Figures 3(a) and 3(b), p < 0.05)

Summary

Introduction

Alzheimer disease (AD) is progressive and neurodegenerative disorder disease among the elderly, characterized by progressive loss of memory and cognition [1]. In China, its rhizome was regarded as folk medicine for the treatment of lung diseases, inflammation, dysentery, traumatic injuries and rheumatism [5]

Methods

Results

Conclusion