Neuroprotective capability of dental pulp stem cells in an in vitro model of Parkinson's disease

Abstract

Event Abstract Back to Event Neuroprotective capability of dental pulp stem cells in an in vitro model of Parkinson's disease Nareshwaran Gnanasegaran1, 2*, Vijayendran Govindasamy2 and Noor Hayaty Abu Kasim1 1 University of Malaya, Department of Restorative Dentistry, Faculty of Dentistry, , Malaysia 2 Hygieia Therapeutics Sdn. Bhd., cGMP-compliant Stem Cells Laboratory, Malaysia Dental pulp stem cells (DPSCs) have been popular since the past decade and extensive amount of work have been carried out to understand them. Their inherent propensity towards neuronal lineage coupled with the fact that they are of neural crest origin has allowed huge emphasis to be placed on DPSCs as a treatment modality for cell replacement therapy (CRT) especially in neurodegenerative diseases. However apart from differentiating into cell lineage of interest, the transplanted cells must also withstand the harsh conditions in that specific microenvironment upon transplantation. In this study, neuroprotective and immunomodulatory capacities of DPSCs were evaluated in an in vitro model of Parkinson’s disease (PD). By using IMR-32 and EOC2 cell lines, we first identified the optimum MPTP concentration to simulate ideal PD model. Following to this, both cell lines were co-cultured with DPSCs and assessed in terms of cell count, viability, DNA damage and levels of inflammatory cytokines. As expected, viability and cell count of both cell lines displayed decrements in dose-dependent manner. However when DPSCs were introduced, the attenuation of neurotoxicity, especially in DNA damage was significant as compared to control (p< 0.05). Additionally, inflammatory cytokines such as ILs, IFN-γ and TNFs were deferentially expressed with the introduction of DPSCs. To conclude, this study has demonstrated the neuroprotective and immunomodulatory properties of DPSCs and these findings are expected to increase our understanding in the application of DPSCs in CRT particularly in those related to neurological concerns. Keywords: Neuroprotection, stem cell, Parkinson’s disease, neurodegeneration, in vitro Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: O01: Postgraduate Travel Awardees Oral Session 1 Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Gnanasegaran N, Govindasamy V and Abu Kasim N (2016). Neuroprotective capability of dental pulp stem cells in an in vitro model of Parkinson's disease. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00082 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Aug 2016; Published Online: 11 Aug 2016. * Correspondence: Mr. Nareshwaran Gnanasegaran, University of Malaya, Department of Restorative Dentistry, Faculty of Dentistry,, Lembah Pantai, Kuala Lumpur, Malaysia, naresh_waran_28@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nareshwaran Gnanasegaran Vijayendran Govindasamy Noor Hayaty Abu Kasim Google Nareshwaran Gnanasegaran Vijayendran Govindasamy Noor Hayaty Abu Kasim Google Scholar Nareshwaran Gnanasegaran Vijayendran Govindasamy Noor Hayaty Abu Kasim PubMed Nareshwaran Gnanasegaran Vijayendran Govindasamy Noor Hayaty Abu Kasim Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.