Neuroprotective activity of Acanthus maderaspatensis L. against colchicine induced Alzheimer’s disease rat model

Abstract

AbstractBackgroundTraditionally, the plants were used for various diseases and disorders, including neuronal disorders. The present study was designed to evaluate the neuroprotective effect of Acanthus maderaspatensis L. against colchicine induced neurobehavioral and biochemical changes in Alzheimer’s disease rat model.MethodNeuroprotective activity was carried out by colchicine induced Alzheimer’s disease on Sprague‐Dawley rats. Test group animals administrated with ethanol extract Acanthus maderaspatensis L. at dose of 200, 400 and 600mg/kg for 28 days. The behavioral parameters were assessed using the Morris water maze test, Digital actophotometer and Plus maze were performed at Days 0, 7, 14 and 28 on intracerebroventricular administration of colchicine for assessing the cognitive and gross behavioral activities in all groups. Biochemical parameter such as lipid peroxidation, glutathione, superoxide dismutase, catalase, total thiols and acetylcholinesterase were assessed and histopathology of brain tissue were assessed on the final day of the experiment.ResultEthanol extract of Acanthus maderaspatensis L. shows a dose dependent neuroprotective activity by significant decrease in time latency of Morris water maze (p<0.001) plus maze (p<0.001) increase in the locomotor activity (p<0.001) in EAM treated group compared to colchicine induced control group. Biochemical parameters showed significant decreases in lipid peroxidation (p<0.001) and increased in superoxide dismutase (p<0.001) catalase (p<0.001) glutathione (p<0.001) total thiols (p<0.001) and acetylcholinesterase level (p<0.001) in the EAM treated groups as compared with colchicine induced control group.ConclusionIn conclusion, the present study suggests that administration of ethanol extract of Acanthus maderaspatensis L. (EAM) possesses neuroprotective activity against colchicine induced cognitive impairment and associated oxidative stress in rats.