Neuropeptide tyrosine-like immunoreactive system in the brain, olfactory organ and retina of the zebrafish, Danio rerio, during development

Abstract

The anatomical distribution of neuropeptide tyrosine (NPY)-like immunoreactivity was investigated in the brain, olfactory organ and retina of the zebrafish, Danio rerio, during development and in juvenile specimens, by using the indirect immunofluorescence and the peroxidase–antiperoxidase methods. In 60 h post fertilization (hpf) embryos, NPY-like immunoreactive cell bodies appeared in the hypothalamus, within the posterior periventricular nucleus. Few positive nerve fibers were found in the hypothalamus and in the tegmentum of the mesencephalon. In 72 hpf embryos, a new group of NPY-like immunoreactive cells was found in the olfactory pit. At day 4 of development, NPY-like immunoreactive cell bodies were detected between the olfactory pit and the olfactory organ. In the hypothalamus the location of positive cell bodies was similar to that reported in the previous developmental stages. A few positive nerve fibers appeared in the tegmentum of the rhombencephalon. At days 7 and 15 of development, the distribution of NPY-like immunoreactivity was very similar to that reported at day 4. However, at day 15, NPY-like immunoreactivity appeared for the first time in amacrine cells of the retina and in nerve fibers of the tectum of the mesencephalon. In 1-month/3-month-old animals, additional groups of NPY-like immunoreactive cell bodies appeared in the glomerular layer of the olfactory bulbs, the terminal nerve, the lateral nucleus of the ventral telencephalic area, the entopeduncular nucleus and in the medial region of the reticular formation of the rhombencephalon. These results show that NPY-like immunoreactive structures appear early during ontogeny of zebrafish. The distribution of the immunoreactive system increases during the ontogeny, the juvenile stages, and reaches the complete development in mature animals. The location of NPY-like immunoreactivity indicates that, during development, NPY could be involved in several neuromodulatory functions, including the processing of visual and olfactory information. In 1-month/3-month-old animals, NPY-like immunoreactive nerve fibers are present in the pituitary, suggesting that, from these stages onward, NPY may influence the secretion of pituitary hormones.