Abstract
Neurokinin A-like immunoreactivity (NKALI) in human cerebrospinal fluid (CSF) was determined by radioimmuno assay (RIA) combined with high performance liquid chromatography (HPLC). the major immunoreactive component did not coelute with NKA, but coeluted with neuropeptide K (NPK), which contains the NKA sequence in its C-terminus. Trypsin treatment of this component from human CSF and of synthetic NPK, produced a substance which coeluted with NKA in the HPLC system. When the NKALI was oxidized with hydrogen peroxide and rechromatographed, the immunoreactivity coeluted with NPK sulfoxide. The results indicate that the main part of the NKALI in CSF is identical with NPK. The mean concentration of NPK measured in CSF from 6 healthy subjects by HPLCRIA was 23 ± 11 (SD) pmol/L.
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