Abstract
A pronounced incorporation of 3H nucleotides into mitochondrial DNA in isolated suspensions of mitochondria has been demonstrated recently for a number of cell types. Although it is generally assumed that the multiplication of mitochondria is accompanied by replication of their DNA, pronounced incorporation of 3H-thymidine into the mitochondria of higher organisms in vivo has yet to be shown. In the present study, uptake has been measured of 3H-thymidine into neurons of mouse spinal ganglia, cockroach nerve cord ganglia and goldfish retina. The frequency of 3H-thymidinc incorporation into neuronal mitochondria precludes in vivo fractionation of a labelled population. Less than 1% labelling of axonal mitochondria is observed. Although neuronal mitochondria have a high half life and several hundred pass a fixed point along an axon in one day in a unidirectional flow, failure to observe a significant mitochondrial uptake leaves open the problem of the nuclcotide pool serving mitochondrial DNA.
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