Neuronal signaling and the regulation of bone remodeling. new insights into the role of neuropeptide Y Y1 and Y2 receptors in osteoblast function

Abstract

Event Abstract Back to Event Neuronal signaling and the regulation of bone remodeling. new insights into the role of neuropeptide Y Y1 and Y2 receptors in osteoblast function Liliana Teixeira1*, Daniela Sousa1, Ana F. Nunes2, Monica Mendes-Sousa2, Herbert Herzog3 and Meriem Lamghari1 1 Universidade do Porto, INEB – Instituto de Engenharia Biomedica, Divisao de Biomateriais, NewTherapies group, Portugal 2 Universidade do Porto, Nerve regeneration group, IBMC - Instituto de Biologia Molecular e Celular, Portugal 3 Garvan Institute of Medical Research, Neuroscience Program, Australia Neuropeptide Y (NPY) has recently emerged as a potential regulator of bone homeostasis. However, the relevance of NPY’s role in osteoblast activity and the biological functions involving NPY receptors in bone homeostasis remain to be clarified. Here we report that chronically elevated NPY levels leaded to a modulation of the level of Y2 receptor expression marked with a transient down and upregulation according to the stage of osteoblast differentiation. We also show that NPY is a negative regulator of Y1 receptor expression. The pharmalogical activation of Y2 receptor with its agonist resulted in similar effect. Functional analysis also revealed the osteogenic potential of NPY with osteoblast phenotype markers being significantly enhanced in osteoprogenitor cells stimulated by NPY, probably due to the down-regulation of Y1 receptor. In contrasts, these cells exhibit a reduction in calcium deposition in extracellular matrix most likely mediated via Y2 receptor signalling. Furthermore, we show that NPY modulates receptor activator of nuclear factor kB (NF– kB) (RANK) ligand and osteoprotegerin, two key factors regulating bone remodelling. Specifically, NPY inhibits the transcriptional activity of RANKL promoter in osteoprogenitor cells and enhances OPG expression in osteoblasts at early stages of differentiation. However, NPY effect on OPG seemed to be unrelated to Y2 receptor activation. Conference: 11th Meeting of the Portuguese Society for Neuroscience, Braga, Portugal, 4 Jun - 6 Jun, 2009. Presentation Type: Poster Presentation Topic: Neuronal Communication Citation: Teixeira L, Sousa D, Nunes AF, Mendes-Sousa M, Herzog H and Lamghari M (2009). Neuronal signaling and the regulation of bone remodeling. new insights into the role of neuropeptide Y Y1 and Y2 receptors in osteoblast function. Front. Neurosci. Conference Abstract: 11th Meeting of the Portuguese Society for Neuroscience. doi: 10.3389/conf.neuro.01.2009.11.151 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Aug 2009; Published Online: 12 Aug 2009. * Correspondence: Liliana Teixeira, Universidade do Porto, INEB – Instituto de Engenharia Biomedica, Divisao de Biomateriais, NewTherapies group, Porto, Portugal, L.S.MoreiraTeixeira@tnw.utwente.nl Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Liliana Teixeira Daniela Sousa Ana F Nunes Monica Mendes-Sousa Herbert Herzog Meriem Lamghari Google Liliana Teixeira Daniela Sousa Ana F Nunes Monica Mendes-Sousa Herbert Herzog Meriem Lamghari Google Scholar Liliana Teixeira Daniela Sousa Ana F Nunes Monica Mendes-Sousa Herbert Herzog Meriem Lamghari PubMed Liliana Teixeira Daniela Sousa Ana F Nunes Monica Mendes-Sousa Herbert Herzog Meriem Lamghari Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.