Neuronal organization in fly optic lobes altered by laser ablations early in development or by mutations of the eye.

Abstract

The role of afferent and efferent connections in the differentiation of optic lobe interneurons was investigated by using laser ablations of neuronal precursors in the brain of Musca domestica and analysis of two eye mutants of the same species. The first mutant, split eye, had no connections between the retina and the optic lobes. In this case the optic lobes were drastically reduced in volume and the neural organization within the neuropil regions was altered. The other mutant, spindle, had reduced retinae that innervated reduced optic lobes with a normal-appearing orderly arrangement of neurons. In addition disordered neuropil, composed of identified visual interneurons, was found that had no afferent innervation. Three main types of alterations resulting from laser ablations were analyzed. These ablations removed entire neuropil regions or parts of these: (1) removal of the first optic neuropil region (the lamina) resulting in receptor axons projecting directly to the second neuropil (the medulla) and sprouting of medulla neurons toward the receptor layer; (2) removal of one part of the third optic neuropil (the lobula plate) and severe alteration of the other part (the lobula) resulting in sprouting of lobula neurons into the medulla neuropil; and (3) removal of the entire optic lobe resulting in reduction of the volume of the lateral midbrain and photoreceptor axons forming a tangle beneath the retina. Our findings confirm that afferent retinal input is essential for normal differentiation and maintenance of many optic lobe interneurons. Furthermore, it was seen that a normal columnar organization of the neuropils and the dendritic patterns of visual interneurons are dependent on afferent inputs. A common response to removal of inputs was a reorganization of axonal and dendritic projections.