Neuronal localization and evoked release of norepinephrine in the cnidarian Renilla koellikeri

Abstract

AbstractNeuronal release of norepinephrine (NE) in the sea pansy Renilla koellikeri was investigated by searching for evidence 1) of neuronal localization of endogenous NE and 2) of synaptic‐like release of exogenously supplied NE in sea pansy tissues. Measurements of endogenous NE by high‐performance liquid chromatography with electrochemical detection corresponded with the visualization of specific NE immunoreactivity in six individual colonies thus sampled. Peroxidase‐antiperoxidase immunohistochemistry with antisera against NE‐aldehyde‐protein conjugates revealed that cellular NE immunostaining was predominantly associated with neurons and amoebocytes constituting the mesogleal nerve net. Transient increases of tritium outflow above background levels were evoked by field electrical stimulation of colonial (rachis) tissues pre‐loaded with 1 μM [3H]NE, under conditions that elicited nerve net‐mediated bioluminescence. This evoked release was largely reduced or abolished in a reversible manner by substituting high‐magnesium or calcium‐free seawater to normal seawater perfusate. Evoked release was significantly enhanced by exogenous norepinephrine (50 μM) and reduced in half or less by the α‐adrenergic blockers phentolamine and yohimbine (10 μM), thus providing support for the existence of an autoregulatory mechanism associated with release. These results suggest that neuronal, synaptic‐like release of NE occurs in the nerve‐net of the sea pansy, a representative of the phylum (Cnidaria) in which the first nervous systems of multicellular organisms are believed to have originated. © 1995 Wiley‐Liss, Inc.