Neuronal and inducible nitric oxide synthase upregulation in the rat medial prefrontal cortex following acute restraint stress: A dataset.

Jereme G Spiers,Conrad Sernia,Nickolas A Lavidis,Hsiao-Jou Cortina Chen,Johnny K Lee

doi:10.1016/j.dib.2016.01.005

Abstract

This data article provides additional evidence on gene expression changes in the neuronal and inducible isoforms of nitric oxide synthase in the medial prefrontal cortex following acute stress. Male Wistar rats aged 6–8 weeks were exposed to control or restraint stress conditions for up to four hours in the dark cycle after which the brain was removed and the medial prefrontal cortex isolated by cryodissection. Following RNA extraction and cDNA synthesis, gene expression data were measured using quantitative real-time PCR. The mRNA levels of the neuronal and inducible nitric oxide synthase isoforms, and the inhibitory subunit of NF-κB, I kappa B alpha were determined using the ΔΔCT method relative to control animals. This data article presents complementary results related to the research article entitled ‘Acute restraint stress induces specific changes in nitric oxide production and inflammatory markers in the rat hippocampus and striatum’ [1].

Highlights

Neuronal and inducible nitric oxide synthase upregulation in the rat medial prefrontal cortex following acute restraint stress: A dataset
Male Wistar rats aged 6–8 weeks were exposed to control or restraint stress conditions for up to four hours in the dark cycle after which the brain was removed and the medial prefrontal cortex isolated by cryodissection
Method relative to control animals. This data article presents complementary results related to the research article entitled ‘Acute restraint stress induces specific changes in nitric oxide production and inflammatory markers in the rat hippocampus and striatum’ [1]

Summary

Data accessibility

Figures Real-time PCR (QuantStudioTM 6 Flex Real-Time PCR System, Applied Biosystems, Foster City, CA) Analyzed Male Wistar rats were subjected to acute restraint stress for 0 (control), 60, 120, and 240 min in the dark-cycle. The first genomic data demonstrating a single acute stress is capable of inducing increased neuronal NOS mRNA expression. The first regional observation of simultaneous increases in neuronal and inducible NOS mRNA in the medial prefrontal cortex. These data provide evidence for rapid inhibition of the inflammatory NF-κB pathway in the medial prefrontal cortex. We have presented genomic data from the medial prefrontal cortex in support of the research article entitled ‘Acute restraint stress induces specific changes in nitric oxide production and inflammatory markers in the rat hippocampus and striatum’ published in Free Radical Biology and Medicine [1]. We have shown data for the mRNA levels of neuronal and inducible isoforms of NOS in addition to the inhibitory subunit of NF-κB (Nfkbia) relative to control animals (Figs. 1–3)

Experimental animals

Habituation and transportation

Treatment protocol and tissue collection

Statistical analysis