Neuromuscular Activation of Triceps Surae Using Muscle Functional MRI and EMG

Abstract

The aim of the present study was to clarify the neuromuscular activation patterns among individual triceps surae (TS) muscles during a repetitive plantarflexion using muscle functional magnetic resonance imaging (MRI) (mfMRI) and electromyography (EMG). Six healthy men participated in this study, performing 5 sets of 10 repetitions of a calf-raise exercise bilaterally, unilaterally, and unilaterally with an additional 15% of body-weight load. The transverse relaxation times (T2) in the medial gastrocnemius (MG), lateral gastrocnemius (LG), and soleus (SOL) muscles were measured from mfMR images obtained with the subject at rest and immediately after exercise; integrated EMG (iEMG) activity was recorded from the same muscles during exercise using surface electrodes. There was remarkable correspondence between the changes in T2 values and iEMG activity under the three different workloads for individual TS muscles. In addition, changes in T2 value that occurred as a function of increasing exercise loads were linearly correlated with iEMG activity in the MG (r=0.58, P<0.05) and SOL (r=0.63, P<0.01), but not in the LG. These results suggest that 1) mfMRI signals and iEMG activity correlate with workload in individual TS muscles, 2) mfMRI signals are associated with neuromuscular activity reflected in iEMG in the MG and SOL but not in the LG, and 3) these relationships are associated with neuromuscular and metabolic factors during exercise.