Neurokinin A-Stimulated Phosphoinositide Breakdown in Rabbit Iris Sphincter Muscle

Abstract

Specific [3H]-substance P binding was saturable and of high affinity (KD = 2.5 nM) with a Bmax of 725 fmol/mg protein in the isolated rabbit iris sphincter muscle. The competition for [3H]-substance P binding was in the order of eledoisin > substance P > kassinin > neurokinin B > neurokinin A > physalaemin. In the same preparation, neurokinin A, as well as substance P induced a concentration-related accumulation of [3H]-inositol phosphates (IPs), and the maximum increase was about 200% of the control at 10−4M. [D-Arg1, D-Trp7,9, Leu11]-substance P (SP) and [D-Pro2, D-Trp7,9]-SP (10−3 M) inhibited substance P or neurokinin A (10−4 M)-induced phosphatidylinositol 4,5-bisphosphate (PIP2) hydrolysis significantly. [D-Arg1, D-Pro2, D-Trp7,9, Leu11]-SP (10−3 M) also inhibited neurokinin A (10−4 M)-induced PIP2 hydrolysis significantly. Neurokinin A and substance P produced concentration-related contractions in normal Ca2+-containing medium. The contractile response was weaker in Ca2+-free medium, and there was no response in 0.2 mM EGTA medium. In Ca2+-free medium, the basal level of [3H]-IPs accumulation was smaller than that in normal medium, and neurokinin A and substance P significantly increased PIP2 hydrolysis. In the 0.2 mM EGTA containing medium, neurokinin A and substance P did not stimulate the PIP2 hydrolysis. These results suggest that in the rabbit iris sphincter muscle, there are tachykinin receptors linking to PIP2 hydorolysis and Ca2+ mobilization and that these mechanisms underlie the mechanism for the neurokinin A-induced contractile response, as well as the substance P-induced one.